Bond fluctuation method for a polymer undergoing gel electrophoresis

A new bond fluctuation method for a polymer undergoing gel electrophoresis

We present a new computational methodology for the investigation of gel electrophoresis of polyelectrolytes. We have developed the method initially to incorporate sliding motion of tight parts of a polymer pulled by an electric field into the bond fluctuation method (BFM). Such motion due to tensile force over distances much larger than the persistent length is realized by non-local movement of...

Conducting Polymer Electrodes for Gel Electrophoresis

In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) can be placed between traditional metal electrodes and an electrolyte to mitigate electrolysis in liquid (capi...

Scaling of megabase DNA undergoing gel electrophoresis

We

A method for the electrophoresis of colicines in agar gel.

The design, construction, and use of an apparatus for the electrophoresis of colicines in agar gel is described, as are some preliminary experiments with it. The possibilities of this method in typing strains of colicinogenic organisms and for the study of colicines are also discussed. In 1953, Ludford and Lederer, using a simple apparatus, demonstrated that substances known as colicines consis...

Solid friction and polymer relaxation in gel electrophoresis.